Choose by question

From segmentation to inspectable evidence.

BioNuclei combines a focused nuclear segmentation pipeline with object level measurements and browser based review. The workflow exposes quantitative evidence without turning an unvalidated model into a biological authority.

Available

Find & count nuclei

Boundary U-Net segmentation followed by deterministic instance separation.

Output: labelled mask, overlay, nucleus count
Evidence: explicit nuclear objects
Available

Measure morphology

Quantify nuclear geometry and inspect population distributions.

Measures: area, perimeter, eccentricity, solidity
Shape: circularity, major/minor axes
Available

Measure fluorescence

Summarize fluorescence within each detected nuclear instance.

Measures: mean, minimum and maximum intensity
Output: per-nucleus measurement table
Available

Interactive object review

Inspect the returned overlay with quantitative nuclei rendered as measurement-aware points.

View: overlay and instance mask
Explore: area, intensity, circularity, eccentricity
Available

Spatial ROI review

Draw a temporary rectangle over the analysis view and summarize the nuclei inside it.

Computed: object count, mean area, mean intensity
Persistence: browser-side only for the current result
Available

Evidence report

Package visual evidence, machine-readable measurements, provenance and specialist review.

Bundle: report, CSV, JSON, masks and overlays
Boundary: no clinical diagnosis or unsupported biology
Validation required

Colocalization

Multi-channel overlap statistics require a validated acquisition model and channel-aware validation before public release.

Validation required

Time-series tracking

Tracking requires temporal metrics, identity handling and explicit failure analysis before release.

Validation required

Learned object classification

A public classifier requires a released model and independent validation set. User images are not silently used to train it.

Review workflow

See what the algorithm actually measured.

The Lab now separates prediction, object measurements and interpretation. A scientist can inspect the overlay, view the per-nucleus table, explore measurement-aware spatial patterns and then download the complete evidence bundle.

1 · Predict

Boundary U-Net produces semantic nuclear information.

2 · Separate

Deterministic processing converts predictions into individual instances.

3 · Measure

Geometry and intensity are computed from the returned instances.

4 · Review

Viewer, table, ROI and evidence-constrained report expose the result.

Run the complete workflow.

Upload a supported TIFF or Nikon ND2 in the BioNuclei Analyzer, inspect the returned evidence, download the report and delete the transient server-side job.

Open BioNuclei Analyzer