Find & count nuclei
Boundary U-Net segmentation followed by deterministic instance separation.
BioNuclei combines a focused nuclear segmentation pipeline with object level measurements and browser based review. The workflow exposes quantitative evidence without turning an unvalidated model into a biological authority.
Boundary U-Net segmentation followed by deterministic instance separation.
Quantify nuclear geometry and inspect population distributions.
Summarize fluorescence within each detected nuclear instance.
Inspect the returned overlay with quantitative nuclei rendered as measurement-aware points.
Draw a temporary rectangle over the analysis view and summarize the nuclei inside it.
Package visual evidence, machine-readable measurements, provenance and specialist review.
Multi-channel overlap statistics require a validated acquisition model and channel-aware validation before public release.
Tracking requires temporal metrics, identity handling and explicit failure analysis before release.
A public classifier requires a released model and independent validation set. User images are not silently used to train it.
The Lab now separates prediction, object measurements and interpretation. A scientist can inspect the overlay, view the per-nucleus table, explore measurement-aware spatial patterns and then download the complete evidence bundle.
Boundary U-Net produces semantic nuclear information.
Deterministic processing converts predictions into individual instances.
Geometry and intensity are computed from the returned instances.
Viewer, table, ROI and evidence-constrained report expose the result.
Upload a supported TIFF or Nikon ND2 in the BioNuclei Analyzer, inspect the returned evidence, download the report and delete the transient server-side job.
Open BioNuclei Analyzer